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THE UC BERKELEY EMLThe Electron Microscope Laboratory (EML) is an imaging core and shared resource facility of the University of California at Berkeley. Our mission is to provide high-quality electron microscopy services, education, and training for the UC Berkeley campus, other academic institutions, and private companies at-large. The lab is equipped with high resolution transmission electron microscopes, scanning electron microscopes, high pressure freezer and full suite of ancillary sample preparation equipment to fit most EM-related needs. The equipment can provide nanometer scale analysis for biological, chemical and materials science. The EML offers multiple levels of service that correspond with the services and training each user desires:
All services are available to anyone with an interest in electron microscopy: graduate and undergraduate students, post-doctoral researchers, faculty, staff, and non-UC users. Please note, the EML now provides fee-for-service full service microscopy for campus and external users as our staff availability allows on an ad hoc basis. Use the Quotation form to request this service. In addition to providing training as a service, the EML supports two MCB courses: MCB 481 B and 481 C. We encourage any UC Berkeley student working with a mentor on campus (undergrad or graduate level) to talk with us about taking the course. It is a great way to get trained to use the equipment in the facility. See the MCB course section for more information. LOCATIONOur address Electron Microscope Lab Phone: 510-642-2085 To find us on campus: We are in Room 21 of Barker Hall, which is located at the ground floor of the building. See the pictures below to help guide you.
You can also find us on Google Maps:
For access on Oxford St:Walk towards the stairs on the corner of Oxford St and Berkeley Way.
Once you reach the top of the stairs turn left towards Barker Hall.
Make Your way towards the door enter on the right door.
Take a slight left towards the elevators.
For access on Hearst Ave:Walk towards the loading dock
Walk down the dock to the left.
Then again to the left is the door to Barker hall. If you dont have key card access either go to the main front entrance or Call the lab at: (510) 642-2085
For detailed written instructions on how to get to our facility, please click HERE. EML STAFFDanielle Jorgens is the director of the EML. She earned her PhD from UC Berkeley (2013) in Comparative Biochemistry, where she trained in advanced sample preparation techniques including cryopreservation for cells and tissues. She was a post-doctoral fellow and then a research assistant professor specializing in the ultrastructure of cancer biology at Oregon Health and Science University (OHSU). Her research interests focus on the ultrastructure of the nucleolus and nucleus in cancer and normal tissues. She is also interested in developing novel methods and applications for volume electron microscopy (Volume EM, FIB-SEM, SBF-SEM) and correlative light and electron microscopy (CLEM). Email: dmjorgens@berkeley.edu Reena Zalpuri earned Masters and Bachelors degrees in Biophysics from Punjab University Chandigarh, India. She had training in transmission electron microscopy while working on her research project titled “Adriamycin treated rat myocardium-light & Transmission electron microscopic study”. She oversees the training and operation of the TEMs within the EML and teaches the TEM course (MCB 481B). Email: jubu@berkeley.edu Misun Kang has expertise in various imaging and analysis techniques including light, electron microscopy, X-ray micro computed tomography, and X-ray fluorescence microscopy through graduate studies at UTK and postdoctoral training at UC Davis and UCSF. Misun has conducted extensive research on normal and pathological mineral formation in various tissues and organs such as teeth, bone, kidney stone, pulp stone, and peyronie’s plaque using correlative microscopy techniques. And now, as a new member of EML, Misun is eager to support researchers and students from a wide range of disciplines in their quest for new knowledge and discoveries. When not in the lab, Misun enjoys exploring the great outdoors by indulging in hiking and camping. Email: misunkang@berkeley.edu Current Student AssistantsNatalie Chaplin Christina Lu Ella Ullrich Even Jorgens General Inquiries and Questions:For inquiries or questions regarding EML recharges or other billing info, please send email to the following address, or use our Contact Us form, or call our main lab phone. Email: ucbeml@berkeley.edu Phone: (510) 642-2085
FORMER EML STAFFGuangwei Min earned his PhD in Structure Biology from Peking University, China. His postdoc training was focused on studying the three-dimensional (3D) structure of biological macro-molecules at New York University. He oversaw the training and operation of the SEMs within the EML, maintained the website, and taught the SEM course (MCB 481C). Kent McDonald was the director of the EML from 1993 to when he retired in 2017. He earned a degree in Botany from UC Berkeley (1972) and did his post-doctoral training in cell biology at the University of Colorado, Boulder. His primary research interest was the ultrastructure of the mitotic spindle apparatus. In recent years, he became interested in development and application of cryopreservation techniques for high resolution EM and EM immunocytochemistry. Dr. McDonald continues to be active in the EM field and is an avid photographer. Email: klm@berkeley.edu Gordon Vrdoljak is a 22 year veteran in analytical chemistry and earned a PhD in Chemistry from the Department of Environmental Sciences at UC Berkeley. He was a Senior Research Associate within the EML at UC Berkeley, where he taught classes in electron microscopy. Following his time with the EML, Dr. Vrdoljak became a Research Scientist IV in the Food and Drug Laboratory at CDPH, a Research Scientist Supervisor of the Chemistry Section of the same Food and Drug Laboratory, and most recently, the Research Supervisor of the Cannabis Testing Section of the Food and Drug Laboratory at CDPH. He has a passion for science and in his spare time enjoys science fiction, ice skating with his daughters, and photography. He has a blog of science images at spicnspan.blogspot.com. Email: Gordon.Vrdoljak@cdph.ca.gov Former Student AssistantsMeera Iyer Haley Rodriguez Caitlin Baxter Shreya Ramasubban Rithika Vatturi Ashmitha Keshavan Kate Ye Karen Zhai Silver (Xinyi) Shi HOW TO CITE THE EMLThe EML strives to provide excellent service to users and students and in-turn we hope that data collected in our facility enables excellent science. For data collection or sample preparation conducted in our facility, by the user or our staff, we ask that the EML be acknowledged in the manuscript, publication or other media. If a particular staff member assisted you, please acknowledge them specifically. **When publishing data from our Zeiss XB 550 and Leica instruments, please cite NIH S10 Grant #S10OD030258-01 in acknowledgments. An example of a general acknowledgement: “Thank you to the staff at the University of California Berkeley Electron Microscope Laboratory for advice and assistance in electron microscopy sample preparation and data collection.” Further, we would like to highlight and elevate the profile of our user’s work and as such request that any publications be shared with us electronically so that we can link to them from our website. See our curated list of publications HERE. We also appreciate that not all data makes it into a publication and we are always open to highlighting images collected by our users on our website. If you have data that you wish to have highlighted in our GALLERY, please let our staff know! Email: ucbeml@berkeley.edu |
EML@UCB News Feb 26th 2025 Our Zeiss Crossbeam 550 FIB-SEM and Leica cryo-suite are available for training and usage! Please inquire with Misun Kang - misunkang@berkeley.edu - directly to arrange training.We also offer use and training on our Leica GP2 Plunge Freezer for cryo-preparation of cells, viruses, bacteria, archaea, & proteins. For larger cells or tissues, or whole organs and organisms we offer use and training on our Baltec HPM 010 High Pressure Freezer for Waffle Method preparations. Our Leica CryoCLEM cryo-fluorescence microscope is also available for use and training to screen for fluorescent ROIs (regions of interest) in your cryo-samples. Feb 26th 2025 The EML will have the following closure dates, please adjust your experiment planning accordingly! Independent users with building and EML access can continue to work in the lab as they need. CLOSED (independent users can access): NEW CHANGES TO THE FTP SERVER!! AS OF AUGUST 1ST 2024 USER DATA WILL NOW BE DELETED AFTER ONE MONTH!!There has been changes to the amount of time user data will be kept on the FTP server. If you have not retrieved your data, we suggest you do that NOW!To access your data please either visit us In Person or go to the FTP Server Page. Aug 1 2024 Please welcome our new Safematic CCU-10 Sputter Coater, Our new Tousimis AutoSamdri 931 Critical Point Dryer and our new Leica EM UC6 and UC7 Microtomes!
Please contact: Misun Kang (misunkang@berkeley.edu) for training on the new Sputter Coater and Critical Point Dryer. Reena Zalpuri (jubu@berkeley.edu) or Danielle Jorgens (dmjorgens@berkeley.edu) for training on our new Microtomes. Sept. 17 2021 Wishing Dr. Guangwei Min a Wonderful Retirement!
Our beloved Guangwei - truly a master of all things in the EML - is retiring from the EML after 15 amazing years. He joined the EML in 2006, recruited from NYU out of his post-doc studying extracellular matrix proteins with cryoEM. We are going to miss his calm and reassuring nature here at the EML; along with his ability to fix everything from IT systems to changing fuses to repairing pumps. Guangwei, we wish for you a long and joyful retirement! Thank you for all you've done for the EML, we will miss you! Love, the EML Staff If you'd like to send Guangwei a message, his min@berkeley.edu will be active during his retirement. Jun. 20, 2020 EML June 2020 Newsletter
Mar 16, 2020 EML has been closed to users due to the Covid19 Public Health Shelter-In-Place Mandate for Alameda County. Check our twitter account for the most recent updates. https://twitter.com/eml_ucb |
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