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	        SEM MICROWAVE TISSUE PROCESSING PROTOCOL




A)	Check water levels in load coolers.
B)	Turn on the load cooler pump prior to microwave use.
C)	Heat the water in the microwave (2 min on HIGH).

Step		Temp. 	Processing Time		Notes
1) Fixation     37	1 or 2 X 40 sec.	600 ul in eppendorf
						Cool to 20 on ice
						Probe in load cooler

2) Buffer rinse	RT	2 X 2.5 min.

3) Osmium fix	37	1 X 40 sec.		Cool to 20 on ice
						Probe in load cooler

4) Buffer rinse	RT	1 X 30 sec.

5) Dehydration					Transfer to baskets
 50%    ETOH  	45	2 X 40 sec.		Baskets in petri dish
						Fill petri 3/4 full
 70%    ETOH	45	2 X 40 sec.		Probe in sol'n 4 mm
						Change the water in
 90%    ETOH	45	2 X 40 sec.		the 2nd beaker
	 					when it's hot to the
 100%  ETOH	45	3 X 40 sec.		touch.