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		     SEM  GENERIC PROCESSING PROTOCOL


 1)	Fixation					1-2 hrs
	2% glutaraldehyde in 0.1M Sodium cacodylate			
	buffer, pH 7.2

 2)	Rinse			3 X 15 min		45 min
	0.1M sodium cacodylate buffer, pH 7.2

 3)	Post-Fix					1-2 hrs
	1% Osmium tetroxide in 0.1M sodium cacodylate buffer, pH 7.2

 4)	Rinse			3 X 5 min		15 min
	0.1M sodium cacodylate buffer, pH 7.2

  5)	Dehydrate 	
	 35%  ETOH				10 min
	 50%  ETOH				10 min
	 70%  ETOH				10 min
	 80%  ETOH				10 min
	 95%  ETOH				10 min
	100% ETOH				10 min
	100% ETOH				10 min

 6)	Critical Point Dry

 7)	Mount specimens onto stubs, using silver paint or paste or carbon dots.
	If you use paint or paste, it must dry overnight (at least) before going
	into the Sputter Coater.

 8)	Sputter Coat.
	The thickness you use can vary between 15 and 30 nanometers.  Most
 	people use between 20 and 25 nanometers.

 9)	SCAN AWAY.