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		   LR White Microwave Processing


A)	Check the water levels in the load coolers.
B)	Turn on the load cooler pump prior to using the microwave.
C)	Heat the water in the microwave (2 min. on HIGH).


Step		Temp. 	Processing Time		Notes

1)Fixation    	37	1 or 2 X 40 sec.	600 ul in eppendorf.
(2% paraformaldehyde,				Let sit 5 min. after MW.
 0.1% glutaraldehyde in				Probe in load cooler.
 0.1 M Sodium cacodylate buffer)

2)Buffer rinse	RT	2 X 2.5 min.	

3)Dehydration (Acetone or Ethanol)		Transfer to baskets.	
						Baskets in petri dish.
 70%	        45	1 X 40 sec.		Fill petri 3/4 full.
						Probe in sol'n 4 mm.
 90%	        45	1 X 40 sec.		Change the water in
						the 2nd beaker when
						it's hot to the touch.

4)Infiltration

 1 : 1	        45	1 X 15 min.		Probe in dish.
						Change the water in
 100% LR White  45	2 X 15 min.		the 2nd beaker when
						it's hot to the touch.

5)  Polymerization
		80	1 X 45 min.		Probe in water dish.	
						Use BEEM capsules.
						Put parafilm under	
						the caps to seal shut.