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					PLT/ LR Gold


1)Fix samples   2% paraformaldehyde	4	  hrs to days
		0.1% glutaraldehyde
		in 0.1 M Sodium cacodylate buffer, pH 7.2

2)	Dehydration-
		30% ETOH		4		15 min
		50% ETOH		4		15 min
		50% ETOH	      -20		45 min
		70% ETOH	      -20		45 min
		90% ETOH              -20		60 min
               100% ETOH              -20    3 X 20 =	60 min

3)	Infiltration-
		3:1  ETOH: LR Gold    -20		60 min
		1:1  ETOH: LR Gold    -20		60 min
		1:3  ETOH: LR Gold    -20		60 min
	100% LR Gold w/o initiator    -20	      overnight
	100% LR Gold w/ initiator     -20		60 min

4)	Embed-	
	100% LR Gold + initiator      -20		24 hrs
	into BEEM capsules

5)	Final Curing	       		RT		3 days



Initiator=	Benzil 0.1% w/v
		 or
		Benzoin methyl ether 0.5% w/v