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Equipment Desciption and Operation Instructions

Hitachi S-5000 Scanning electron microscope
The Hitachi S-5000 is a high resolution, cold field emission SEM. It operates from 0.5 to 30 kV, with a magnification range of 30X to 800,000X. Image resolutions of 0.6nm (at 30kV) and 3.5nm (at 1kV) are possible with secondary electron images.

Please note that samples are limited in size for use in this microscope. We can accomodate samples up to 9.5mm x 5mm x 2.4 mm (height).
S-5000 Standard Operating Procedure
S-5000 startup - for use by EML Staff only.
Procedures to convert stereo pairs to RED/CYAN anaglyphs with photoshop.
FEI Tecnai 12 Transmission electron microscope
The Tecnai 12 is an advanced transmission electron microscope with accelerating voltages from 20-120kV. It offers magnification ranges from 120X to 300,000X. The microscope has a lens design to optimise low contrast imaging for biological specimens. It has a cryo stage for viewing frozen specimens. The staff keeps it in optimal working condition so the user does not have to do any aligning. It is easy to use and has a photographic capacity of 50 negatives. It also has a low resolution digital camera.

Tecnai 12 TEM Standard Operating Procedure
EML staff only notes

JOEL 1200 EX Transmission electron microscope

This instrument offers the highest basic performance as a 120 kV transmission electron microscope employing a uniquely designed 3-stage 6-lens imaging system. It offers operational ease, good image quality and high resolution at low to high magnifications. 

Hitachi TM-1000 Scanning Electron Microscope
The TM-1000 utilizes scanning electron microscope (SEM) technology. It is easy to use while retaining powerful imaging capabilities. Surface morphology is shown in stereoscopic detail with images in contrast due to different average atomic number composition within the sample.
It provides a real alternative to optical microscopes, stereo microscopes and confocal laser scanning microscopes. It has applications for many sectors including life science, food, cosmetics, healthcare, pharmaceutical, textiles, materials science, semiconductor and education.

Hitachi TM-1000 SEM Standard Operating Procedure

Balzers HPM 010 High Pressure Freezer
The Bal-Tec HPM 010 is a high pressure freezing machine that is designed to freeze non-cryoprotected samples up to 0.5mm in thickness without significant ice crystal damage. Samples can then be processed for TEM using the freeze substitution protocol. These two methods are the state-of-the-art for TEM specimen preparation.

For instructions about High Pressure Freezing, please go to www.cryoem.org.
RMC MTX, Reichert Ultracut E, RMC MT6000 Microtomes

Several microtomes and a LKB glass knife maker, are available for making both thin and thick sections of samples. We don't supply diamond knives, but allow users to bring their own for sectioning.

Tousimis AutoSamdri 815 Critical Point Dryer

Tousimis AutoSamdri 815, Series A, Critical Point Drying Aparatus
The AutoSamdri is a critical point drying apparatus for sample prepartion in the electron microscope laboratory. It is used in dehydration of specimens by exchange of ethanol with liquid carbon dioxide.

Critical Point Dryer Standard Operating Procedure
Bal-tec MED 020 Suptter Coater

MED 020 Bal-tec Modular High Vacuum Coating System
The MED 020 coating system currently supports high resolution sputter coating of specimens with Au,Pt, Au/Pd, and Cr metals.

Med 020 Standard Operating Procedure

Tousimis Sputter Coater

The tousimis sputter coater sitting on top of the Bio-Rad E5400 controller is an easy to use machine which can coat Au/Pd.

Standard Operating Procedure

BIO-RAD E5400 Sputter Coater (not available now)

BIO-RAD E5400 Sputter Coater
The E5400 sputter coater is a DC diode sputter coater with magnetic deflection for coating SEM specimens with an ultrafine metallic coating.

Sputter Coater Standard Operating Procedure

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