| Schedule for the Berkeley CryoEM Course | |||||
| Jan. 7 - 12, 2007 | |||||
| Saturday, Jan 7 | Room | Group | Event | ||
| 7:00 - 9:00 | Durant Hotel | Registration, Welcome Reception | |||
| Outline of course, Introduction of students, | |||||
| faculty, manufacturers | |||||
| Sun. Jan 7 | Room | Group | Event | Instructors | Technical Asistants |
| 8:00 - 8:30 | ABC | Orientation | Kent McDonald | ||
| 8:30 - 9:30 | ABC | Introduction to HPF - Freeze Substitution | Kent McDonald | ||
| 9:30-10:15 | Vitreous Cryosectioning and Diamond Knife Use | Helmut Gnaegi | |||
| 10:15 - 10:30 | break | ||||
| 10:30 -1:00 | A | HPF - Baltec HPM 010 | Kent McDonald | ||
| B | HPF - Leica EMPact | Paul de George | |||
| C | HPF - Wohlwend HPF-01 | Johnny Hagen | |||
| 1:00 - 2:00 | lunch | ||||
| 2:00 -4:30 | A | HPF - Wohlwend HPF-01 | Johnny Hagen | ||
| B | HPF - Baltec HPM 010 | Kent McDonald | |||
| C | HPF - Leica EMPact | Paul de George | |||
| 4:30 - 5:00 | ABC | regroup | |||
| 5:00 - 6:30 | Dinner | ||||
| 6:30 -9:00 | A | HPF - Leica EMPact | Paul de George | ||
| B | HPF - Wohlwend HPF-01 | Johnny Hagen | |||
| C | HPF - Baltec HPM 010 | Kent McDonald | |||
| 9:00 - 9:30 | ABC | Begin Freeze Substitution & Tokuyasu Infiltration | Kent McDonald - Paul DeGeorge | ||
| Mon. Jan 8 | Room | Group | Event | Instructors | |
| 8:30 - 9:15 | ABC | Cellular Tomography | Eileen O'Toole | ||
| 9:15 - 10 :15 | ABC | The Tokuyasu Technique | TBA | ||
| break | |||||
| 10:30 - 5:00 | A | Introduction to Tomography | Eileen O'Toole | ||
| B | Cryo-ultramicrotomy - Tokuyasu | TBA | Jan Leunissen, Hong Yi | ||
| C | Vitreous Cryosectioning and Diamond Knife Use | Helmut Gnaegi | |||
| ~12:15 - 1:00 | lunch | ||||
| 5:00 - 5:30 | regroup | ||||
| 5:30 - 9:00 | Winery tour and dinner | ||||
| Tues. Jan 9 | Room | Group | Event | Instructors | |
| 8:30 - 10:30 | ABC | Microwave Processing | Elaine Humphrey | ||
| 10:30 - 5:00 | A | Cryo-ultramicrotomy - Tokuyasu | TBA | Jan Leunissen, Hong Yi | |
| B | Vitreous Cryosectioning and Diamond Knife Use | Helmut Gnaegi | |||
| C | Introduction to Tomography | Eileen O'Toole | |||
| ~12:15 - 1:00 | lunch | ||||
| 5:00 - 5:30 | regroup | ||||
| 5:30 - 7:00 | Dinner Break | ||||
| 7:00 - 10:00 | ABC | infiltration and embedding tissue | Kent McDonald - - Paul DeGeorge - others | ||
| Weds. Jan 10 | Room | Group | Event | Instructors | |
| 8:30-3:00 | A | Vitreous Cryosectioning and Diamond Knife Use | Helmut Gnaegi | ||
| B | Introduction to Tomography | Eileen O'Toole | |||
| C | Cryo-ultramicrotomy - Tokuyasu | TBA | Jan Leunissen, Hong Yi | ||
| ~12:15 - 1:00 | lunch | ||||
| 3:00-4:30 | ABC | Participants porject discussion | Jan Leunissen | ||
| 4:30-6:30 | ABC | Immunogold practical - Primary ab | Hong Yi | ||
| Thurs. Jan 11 | Room | Group | Event | Instructors | |
| 8:30 -9:30 | ABC | Immunogold practical - secondary ab | Hong Yi | ||
| 9:30-11:00 | ABC | Immunogold lecture | Jan Leunissen | ||
| 11:00-1:00 | ABC | Immunogold practical: wash and silver enhancement (on-grid) | Hong Yi | ||
| 1:00-3:30 | ABC | Immunogold lecture (can eat lunch during lecture) | Jan Leunissen-Hong Yi | ||
| 3:30- 6:00 | ABC | Immunogold practical: silver enhancement (pre), scoping (on-grid) | Hong Yi | ||
| 7:00 - 9:00 | Dinner at Skate's on the Berkeley waterfront | ||||
| Fri. Jan 12 | Room | Group | Event | Instructors | |
| 8:30 - 10:00 | C | Microscopy of all samples | |||
| 10:00 - 11:30 | B | Microscopy of all samples | |||
| 11:30 - 1:00 | A | Microscopy of all samples | |||
| 1:00 - end | ABC | Lunch, General Discussion and Presentation of Results | |||